Particle Stiffness and Surface Topography Determine Macrophage‐Mediated Removal of Surface Adsorbed Particles

Cellular surface recognition and behavior are driven by a host of physical chemical features which have been exploited to influence particle–cell interactions. Mechanical topographical cues define the milieu plays an important role in defining range cellular activities such as material recognition, adhesion, migration through cytoskeletal organization signaling. In order elucidate effect local mechanical generated adsorption particles underlying on primary human monocyte-derived macrophages (MDM), series poly(N-isopropylacrylamide) (pNIPAM) with differing rigidity self-assembled form defined particle-decorated surface. Assembly surfaces is facilitated modification glass possess positive charge functionalization using 3-aminopropyltriethoxysilane (APTES) or coating poly(L-lysine) (PLL). MDMs noted preferentially remove higher degrees crosslinking (stiffer) than those lower (softer). Alterations density enabled greater area be cleared. Uniquely, impact particle evinced direct surface, suggesting novel approach for controllably affecting cell-surface response.